The in vitro effects of n-3 fatty acids on immune response regulation of bovine ex vivo endometrial explants

Fatty acids are considered metabolic intermediaries, although new facts indicate they also work as signaling molecules with different roles in the immune response. Based on that, this study, we investigated anti-inflammatory effects of n-3 polyunsaturated fatty (PUFAs) eicosapentaenoic acid (EPA), docosahexaenoic (DHA), and α-linolenic (LNA) ex vivo bovine endometrial explants. For this, two groups were formed: (1) LPS-challenged (2) control, both to evaluate accumulation proinflammatory cytokines interleukin 1β (IL1B) 6 (IL6). To develop female reproductive tracts from non-pregnant Angus heifers without evidence diseases selected. Endometrial explants processed treated for 24 h EPA, DHA, LNA five concentrations (0μM, 50μM, 100 μM, 200μM 400 μM) then, challenged LPS h. Supernatants collected concentration IL1B IL6 by ELISA. Explants EPA control reduced ILB (200µM) (400 µM), (50 µM; µM) group. DHA decreased at 200 µM group, In contrast, only 400μM (from groups). conclusion, is best anti inflammatory option decrease pro-inflammatory (IL1B IL6) explants; while be last promote an